Transient Transfection Service

Creative Bioarray has rich experience in transient transfection and cell culture, and has rich experience in the construction of transfection vectors and cell culture after transfection. Our platform can help researchers who need transient transfection experiments for protein expression verification and gene function research to speed up their turnover. We have established a mature agreement and can provide instant transfection technical services with fast turnaround time according to your requirements.

Transfection

Transfection is the process of experimentally delivering exogenous DNA, RNA, synthetic polynucleotides or oligonucleotides to cultured cells without the use of viral infection. Transfection enables genome, transcriptome and proteomic studies of gene expression, gene modification (including gene editing), RNA transcription or RNA interference. Different transfection methods are suitable for different needs and help customers achieve successful results.

Stable Transfection

Stable transfection means that foreign DNA is transported to the nucleus, but not integrated into the genome. Stable transfection produces long-term gene expression, which requires screening and cloning of cells for larger-scale protein production. This can minimize the differences between batches for long-term pharmacological research, gene therapy or to determine long-term gene regulation mechanisms.

Transient Transfection

Transient transfection means that foreign DNA enters the nucleus through the cell membrane and nuclear membrane, integrates into the host genome and continues to express. Transient protein expression in mammalian cells does not integrate the expression vector cloned with the foreign gene into the host cell genome to express the foreign protein, and the recombinant protein can be quickly prepared in a short time. Transient expression technology is widely used in the development of recombinant monoclonal antibodies and recombinant protein drugs.

Schematic diagrams of two different transfections.Fig 1. Schematic diagrams of two different transfections. (Kim T K, et al. 2010)

Transiently transfected genetic material will be lost due to environmental factors and cell division, so the choice between stable transfection or transient transfection depends on the purpose of the experiment.

Transient Transfection Services

Transient expression technology can quickly prepare recombinant proteins in a short time and is widely used in the development of recombinant monoclonal antibodies and recombinant protein drugs. Compared with stably transfected genes, transiently transfected genes are only expressed for a limited time and will not be integrated into the genome. We use mammalian cells and insect cells as the main transient expression cells, and obtain a large number of expression products in a short period of time through high-density fed-batch (FB) culture. Plasmid DNA, siRNA, RNAi duplex, oligonucleotide and RNA can be transfected into eukaryotic cells. Transiently transfected cells are usually harvested 2-4 days after transfection and can be used for specific gene-encoded protein products and short-term gene RNA expression. In addition, it can also be used for RNA interference (RNAi) in gene silencing, or for the production of small-scale recombinant protein manufacturing batches. In many drug discovery applications, it is beneficial to use transient transfection methods to quickly screen protein constructs with correctly folded and/or glycosylated proteins, which allows simultaneous evaluation of various candidate molecules in less than a week.

Our transient transfection services

Transient transfection vector construction

This service is mainly for the construction of expression vectors used in transient transfection, as well as some related technical services.

Cell culture and transient transfection

This service is for the transient transfection and cell culture of the constructed expression vector. We also provide a combined service from the vector construction to the whole process of transfection.

Technical Advantages

  • Able to quickly produce small to medium amounts of recombinant protein
  • Low experiment cost
  • One host can carry multiple copies, and the expression efficiency is high

Creative Bioarray provides a variety of cell technology services from transfection vector construction to transiently transfected cell culture, helping our customers speed up experimental turnaround. You will benefit from our technical expertise and state-of-the-art facilities, and our scientific team can work with you to find the best solution.

If you are interested in our services or have any specific needs, please feel free to contact us. We look forward to working with you in the near future.

References:

  1. Kim T K, Eberwine J H. Mammalian cell transfection: the present and the future[J]. Analytical and bioanalytical chemistry, 2010, 397(8): 3173-3178.
  2. Dyson M R. Fundamentals of expression in mammalian cells[J]. Advanced Technologies for Protein Complex Production and Characterization, 2016: 217-224.
For research use only. Not for any other purpose.

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