Creative Bioarray can successfully implement a number of technical services for in vitro analysis of angiogenesis, including tube generation analysis, sprouting analysis, endothelial cell function analysis, and so on. The cell culture method based on a basement membrane-like matrix is conducive to the development of in vitro angiogenesis analysis and testing. We have formed a mature test protocol that can realize the analysis of human endothelial cell lines and animal primary cells.
Angiogenesis is an important process of normal tissue development and wound healing, but it is also related to a variety of pathological conditions. The mechanisms that contribute to angiogenesis include endothelial migration, proliferation, sprouting, branching, differentiation, and lumen formation. The imbalance of blood vessel growth may affect major diseases such as stroke, myocardial infarction, arteriosclerosis, and arthritis. At the same time, tumor vascularization is one of the hallmarks of cancer. Angiogenesis analysis can provide insight into the pro-angiogenic or anti-angiogenic potential of various drugs, thereby helping to develop new treatment methods. The angiogenesis analysis can measure angiogenesis in vitro in a fast and quantifiable way. Based on existing knowledge, in vitro detection principles include the proliferation, migration, differentiation of endothelial cells, co-culture with parietal cells and fibroblasts, and blood vessel growth in organ culture. The current popular angiogenesis analysis is usually constructed by mixing primary or immortalized endothelial cells with conditioned medium and spreading them on the basement membrane matrix.
Fig 1. The angiogenic switch in carcinogenesis. (Rahman H S, et al. 2020)
Angiogenesis analysis is a widely used in vitro technique for obtaining angiogenesis in a simple, cost-effective, and reproducible manner. These technologies are the basis for determining the cellular effects of some new anti-cancer compounds or therapies and studying the biochemical and molecular mechanisms of angiogenesis in cancer. Our angiogenesis test service conducts angiogenesis analysis for endothelial cell function and cancer-related research. Using in vitro techniques related to angiogenesis, we provide tube formation analysis, sprouting experiments, and other Angiogenesis related analysis tests. The standardized process of this service includes substrate selection, cell seeding, live cell imaging, and data analysis. We accept analysis and testing of human endothelial cell lines (such as HUVEC, HMVEC, HDMEC) or animal primary cells (primary cells isolated from the aortic arch).
Cell proliferation test
Cell migration test
Cell differentiation test
Endothelial-parietal cell co-culture test
Fig 2. Services related to angiogenesis assay.
Creative Bioarray provides endothelial cell-based angiogenesis analysis services. This service uses multiple functional analysis experiments such as cell growth, migration, and adhesion to help researchers gain knowledge about angiogenesis. You will benefit from our angiogenesis in vitro technology expertise and stromal cell culture technology equipment. Our scientific team can work with you to find the best solution to meet your needs. If you have test requirements related to angiogenesis, please feel free to contact us. we will provide you with analysis services with fast turnaround.