Creative Bioarray is able to implement cell line certification STR analysis services to help customers investigate possible intra-species contamination and inter-species cross-contamination. The results of this service can be used to assist journal publication, or to troubleshoot possible human errors in experiments. We have established a mature protocol for STR genotyping to provide you with a one-stop service.
According to the recommendations of the American Type Culture Collection (ATCC) Standards Development Organization Working Group ASN-0002, short tandem repeat (STR) analysis is a standard test for validating cell lines. The analysis of highly variable STRs provides a simple, inexpensive, and specific genetic "fingerprint" of cell lines. Cell line STR analysis confirms cell identity by analyzing DNA microsatellite regions with the variable numbers of repeats and located in the entire genome. The important thing is that STR analysis is the test required by most journals. At present, STR markers for human cell lines have formed a relatively complete genotype profile of cell lines, but the standardization method for STR purification of non-human cells needs to be further improved. Our STR analysis service is mainly carried out for human cell lines, and also accepts STR analysis of cell lines of experimental animals such as mice and rats.
Fig 1. Flow chart outlining recommendations for maintenance of cell line stocks. (Yu M, et al. 2015)
Intraspecies authentication of human cell lines: to verify whether the cell line in question is of human origin by detecting the absence of standard markers;
Interspecies cross-contamination analysis service: Species-specific authentication can detect cross-contamination between common animal types such as pigs, cats, Chinese hamsters, rhesus monkeys, sheep, horses, rats, dogs and cattle, and human cell lines;
Selection of STR Markers
According to the basic source information (species, cell line names, and potentially contaminating cell names) of the cell samples submitted by customers, our experts will use NCBI's BLAST tool to design STR markers. We provide detection of at least 8 STR loci, and customers can choose to increase the number of labels according to the situation.
Design multiple PCR primers according to the STR tags planned in the designed scheme.
We use the internally designed multiplex PCR standardization process to perform PCR amplification on the samples, and obtain the amplicon sequence through multiplex PCR. The resulting amplicons are separated using capillary electrophoresis to determine the number of replicates in the sample.
The STR data of our service can be compared with the STR profiles of some STR databases (ATCC, DSMZ, JCRB, RIKEN, CLIMA, MD Anderson, Sanger). The purpose of this is to verify whether the genotype of the cell line in question is the same as the genotype of the putative parental cell.
Fig 2. The basic process of cell line STR profiling analysis service.
Creative Bioarray provides multiple PCR-based STR gene analysis services to help customers complete cell line certification. You will benefit from our experienced team of scientists to provide you with the best time-effective solution to meet your needs. If you are interested in our services or have any specific needs, please feel free to contact us. We look forward to working with you in the near future.