Flow Cytometry Analysis Services

Creative Bioarray designed and developed flow cytometry analysis services for cell line characterization. We have a complete streaming equipment platform and are equipped with experienced scientists to complete analysis work. This service is based on the high-throughput analysis characteristics of flow cytometry and can accomplish a variety of analysis purposes. We have established an optimized protocol that can provide you with a time-efficient flow cytometry analysis solution.

Flow Cytometry Applications in Cell Line Characterization

Flow cytometry provides an effective method to identify cells in solution. One of its advantages is the ability to perform analytical measurements in a very fast time span. Our flow cytometry service uses advanced flow cytometry technology for analysis. Flow cytometry has a wide range of analytical applications. It has been reported that it can measure cell size, cell granularity, total DNA, newly synthesized DNA, DNA gene expression, surface receptors, intracellular proteins, and transient signals. The principle of flow cytometry is to identify and sort target cells based on the expression of specific cell surface and intracellular markers. The application of flow cytometry analysis in cell line characterization can have many aspects, such as cell proliferation ability, cell cycle, monitoring protein expression of the recombinant expression system, and so on. We developed this service by applying these capabilities of flow cytometry to cell line characterization.

Fig 1. A case of analyzing the productivity of cultured cells by flow cytometry. (Meyer K V, et al. 2021)Fig 1.A case of analyzing the productivity of cultured cells by flow cytometry. (Meyer K V, et al. 2021)

Available Services

Our flow cytometry analysis service aims to provide comprehensive flow cytometry services to help customers solve practical problems in the process of cell line development and characterization. Flow cytometry can be widely used to sort and characterize the molecular characteristics of cell lines. During cell line development, cell clones using fluorescence-activated cell sorting (FACS) have the potential to combine the separation of single cells with sorting based on specific cell attributes related to productivity and/or growth. In this way, cell lines with the desired phenotype are identified for application.

Recombinant cell lines used for research and production require monitoring of the stability and product of recombinant fragments. Our platform can use fluorescent dye-conjugated or unconjugated antibodies to identify targets of interest to customers. Our flow cytometry service department provides the following flow cytometry applications (including but not limited to the following).

  • Assess cell viability in mammalian cell lines;
  • Use flow cytometry to monitor antibody productivity and isolate high-secreting hybridoma cells;
  • Analyze apoptosis by flow cytometry;
  • Stability of monoclonal antibody production in hybridoma cell culture;
  • Measure the intracellular pH value during the process of hybridoma cell culture in batch and continuous culture;
  • Monitor baculovirus infection and protein expression in insect cells;
  • Use protein distribution to analyze budding yeast population structure and cell cycle progression;
  • Characterize bacteria by flow cytometry and evaluate the viability of bacteria;

Advantages of Flow Cytometry

RNA. Targets that can be analyzed include miRNA and mRNA transcripts;

Protein expression. Whether it is located in the cytoplasm, cell membrane, or nucleus;

Post-translational modification of proteins. The targets that can be analyzed by flow cytometry include lysis and phosphorylation of proteins;

Identification and characterization of different cell subpopulations in heterogeneous samples. For example, analysis of different T cell subpopulations;

Cell health status. Flow cytometry can detect apoptotic cells or cell death;

Cell cycle status. Flow cytometry provides a powerful tool for evaluating cells in G0/G1 phase, S phase, G2, or polyploidy, including analysis of cell proliferation and activation;

Fig 2. Flow cytometry application services in cell line characterization. - Bio-microarrayFig 2. Flow cytometry application services in cell line characterization.

Creative Bioarray can complete flow cytometry analysis for different analysis needs in cells, helping our customers save time and effort. The powerful analysis function of the flow cytometer helps customers complete the analysis and sorting of multiple targets. You will benefit from our technical expertise and flow cytometry analysis experience. If you are interested in the above applications of flow cytometry or have other analysis needs, please feel free to contact us. We look forward to working with you in the near future.

References:

  1. Meyer K V, Siller I G, Schellenberg J, et al. Monitoring cell productivity for the production of recombinant proteins by flow cytometry: An effective application using the cold capture assay[J]. Engineering in Life Sciences, 2021, 21(5): 288-293.
  2. Maciorowski Z, Chattopadhyay P K, Jain P. Basic multicolor flow cytometry[J]. Current protocols in immunology, 2017, 117(1): 5.4. 1-5.4. 38.
For research use only. Not for any other purpose.

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