Cell Viability Assays

Creative Bioarray provides a wide selection of cell viability assays to help determine the overall health of cells, optimize cell culture techniques, and evaluate cell survival after treatment with experimental stimuli or compounds.

About Cell Viability

Cell viability is the quantification of the proportion of living and healthy cells within a population. Assessing cell viability, a key step in daily cell manipulation, is often a requirement for subsequent analysis and processing. Cell viability is also important for the estimation of cell proliferation and cytotoxicity. These cell-based assays are important parameters for biological studies and drug development.

Multiple parameters can be used to determine cell viability, such as cell membrane integrity, mitochondrial function and metabolic activity. These parameters can sense different characteristics and provide a visual readout of cell health using fluorescence microscopy, confocal microscopy, microplate reader, or flow cytometer.

Different assays can individually or together form the basis of assessing cell viability. Choosing the best cell viability assay to suit particular needs from a variety of options available requires understanding what each assay measures as an endpoint, the correlation, sensitivity, reliability and compatibility of different measurements with relevant cell types, and the limitations of different assays.

Cell Viability Assays

Cell Viability Assays at Creative Bioarray

A wide selection of cell viability assays is available at Creative Bioarray. These assays can be applied to different cell types and research purposes. Our cell viability assay services include but not limited to the methods summarized in the table below.

Cell Viability Assays Overview Detection Methods
Colorimetric Assays
  • MTT
  • MTS
  • XTT
  • WST-1
  • WST-8
  • CCK-8
  • SRB
  • LDH
  • Natural Red
  • Crystal Violet
Evaluation of cell metabolic activity through the measurement of biochemical markers.
  • Spectrophotometer
Dye Exclusion Assays
  • Trypan Blue
  • Congo Red
  • Erythrosine B
  • Eosin
The simplest method to estimate the proportion of viable cells in a cell population. Viable cells with intact cell membranes exclude dyes.
  • Microscopy
Luminometric Assays
  • Luminescent ATP assay
  • Real-time viability assay
Detecting enzyme activity by biochemical reaction.
  • Microscopy
  • Fluorescence microscopy
Fluorometric Assays
  • CFSE
  • Calcein-AM
  • AlalmarBlue
  • PI/7-AAD
  • Annexin V/PI
  • GF-AFC
Distinguishing dead and viable cells using fluorescent dyes.
  • Fluorescence microscopy
  • Fluorometer
  • Flow cytometer
  • Microplate reader
3D Cell Culture A three-dimensional environment is established to simulate in vivo physiological conditions.  

Creative Bioarray provides sensitive, highly specific and optimized cell viability assays with consistency and reproducibility. Our experienced scientific team is capable of choosing the best assay format and providing the best customized cell viability assays to meet the specific requirements of our customers. We also offer a range of cell viability products for the assessment of cell culture techniques, toxicity of substances and drug screening.

If you are interested in our services or products, please feel free to contact us for further assistance.

References:

  1. Aslantürk Ö S. In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. InTech, 2018.
  2. Riss T L, et al. Cell viability assays. Assay Guidance Manual [Internet]. Eli Lilly & Company and the National Center for Advancing Translational Sciences, 2016.
For research use only. Not for any other purpose.

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As a pioneer of biotechnology, Creative Bioarray has developed into one of the largest independent biotechnology companies in the world. We are committed to providing our customers with innovative high-quality products and services through discovering, developing, and manufacturing.

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