Cell Sorting Services

Creative Bioarray provides comprehensive cell sorting services to meet your different needs in scientific research and drug development.

Introduction of Cell Sorting

Cell sorting is the process of separating a specific cell type from a heterotypic cell mixture based on the different characteristics of each cell type, such as cell size, morphology, and surface protein. It allows for more in-depth characterization and further analysis of specific cells. The isolated cells can be cultured or lysed for a variety of downstream fundamental research, including cell fate, cell function, cell singling, gene expression, protein engineering, cell engineering, etc. Cell sorting has become a valuable tool for biotechnological and physical research. In clinical application, the sorted homologous cell populations may also be used as potential therapeutics or used for drug discovery.

Cell purification methods based on cell surface antigen expression. (Matula T J, et al., 2018)
Figure 1. Cell purification methods based on cell surface antigen expression. (Matula T J, et al., 2018)

The techniques available at present for rapid and accurate enrichment of bulk cells or single cells include fluorescent-activated, magnetic-activated, buoyancy-activated, microfluidic cell sorting, and laser capture microdissection. Cells can be sorted as a population or analyzed at single-cell level. Positive selection and negative selection are two common approaches used in cell sorting. The main difference is that the target cells are labeled and tagged in the positive selection approach, while the unwanted cells are labeled in negative selection approach.

Cell purification methods based on cell surface antigen expression. (Matula T J, et al., 2018)
Figure 2. Overview of single-cell isolation technologies discussed in the section. (Hu P, et al., 2016)

Our Cell Sorting Services

Whether you need bulk cell sorting or single cell sorting, need cell to culture or downstream analysis, we can use different cell sorting techniques to your different requirements.

Fluorescent-Activated Cell Sorting (FACS)

On the basis of flow cytometry, fluorescent-activated cell sorting (FACS) has the ability to sort and collect specific cell subsets or single cells according to the different color of fluorescent labeling for further studies. FACS is a very powerful multi-parameter analysis tool and the most accurate cell sorting technique.

Magnetic-Activated Cell Sorting (MACS)

Magnetic-activated cell sorting (MACS), also known as immunomagnetic cell sorting, is a highly specific technique for bulk cell separation and enrichment. Cells that bind to tiny magnetic beads through antibody interactions can be magnetically isolated. This method is generally applicable to only one separation standard or characteristics.

Buoyancy-Activated Cell Sorting (BACS)

Buoyancy-activated cell sorting (BACS) is a novel cell sorting technique. The targeted cells can bind to antibodies conjugated with microbubbles and then removed through flotation. This method does not cause mechanical damage to cells. It is an effective tool in preclinical research and clinical applications.

Microfluidic Cell Sorting

Microfluidic technology has been recently developed to overcome some of the problems of other cell sorting equipment. It realizes the miniaturization of cell sorting equipment to microchips. Since the initial sample volume is much smaller, this method is suitable for precious samples.

Laser-Capture Microdissection (LCM)

Laser-capture microdissection (LCM) can be used to isolate target cells from a mixed population by utilizing a laser under microscopic visualization. This technique has advantages of high precision and less contamination.

With professional scientists and state-of-the-art technique platform, Creative Bioarray can customize the best solution to provide our customers with high-quality results of cell sorting with fast turnaround time and competitive price. If you need more detailed information, please feel free to contact us. We look forward to cooperating with you.

References:

  1. Matula T J, et al. Ultrasound-based cell sorting with microbubbles: A feasibility study. The Journal of the Acoustical Society of America, 2018, 144(1), pp: 41-52.
  2. Hu P, et al. Single cell isolation and analysis. Frontiers in cell and developmental biology, 2016, 4: 116.
  3. Mattanovich D, & Borth N. Applications of cell sorting in biotechnology. Microbial cell factories, 2006, 5(1): 12.
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