Cell Line Authentication Services

Creative Bioarray provides accurate and reliable cell line authentication services for both human and animal cell lines. We aim to dispel any concerns about the presence of misidentification, cross contamination or genetic instability in the cells that our customers are working with. Our results are authoritative and widely recognized.

Why is cell line authentication important?

As important models for complex biological systems, especially those of human tissues,in vitro cell culture systems are indispensable research tools. Based on the experiments of cell lines that can accurately represent the characteristics of their original sources, meaningful and reproducible tissue-specific conclusions can be drawn. Unfortunately, many cell lines have serious problems of misidentification, cross contamination with other cells, incorrect classification, over subcultured or genetic instability. This will seriously affect the correctness of research findings based on these cells, and serious waste time and laboratory resources. Due to the magnitude of this problem, cell line identification is really necessary for biological research, pharmaceutical industries and clinical applications. Cell line authentication is required for more and more journals and institutions.

Figure 1. Usage of five misidentified cell lines in the scientific literature as shown by PubMed searches for cell line name and incorrect tissue dentity. (Fusenig N E, et al., 2017)
Figure 1. Usage of five misidentified cell lines in the scientific literature as shown by PubMed searches for cell line name and incorrect tissue dentity. (Fusenig N E, et al., 2017)

What is cell line authentication?

Cell line authentication mainly focuses on the following four aspects:

  • Species identification. Short tandem repeat (STR) profiling analysis is the most important method. Other commonly used methods include chromosome analysis, isoenzyme analysis, etc.
  • Identification of tissue of origin. Morphological methods and detection of tissue-specific antigens are usually used approaches.
  • Analysis of cross contamination. Isoenzymology, STR profiling, and karyotype analysis can confirm the presence or absence of cross-contamination in cell cultures.
  • Identification of malignant transformation. Karyotype, cell growth behavior and tumor formation in nude mice are useful indicators.

Our Services

STR Profiling Analysis

Short tandem repeats, also known as microsatellites, are tandemly repetitive DNA sequence elements. In human genome, they are one of the most informative polymorphic markers. STR profiling analysis is considered as the basis of standard for cell line authentication. In particular, it helps ensure the integrity and quality of human cell lines.

Karyotyping

Karyotyping is the process of artificially staining, pairing, numbering, grouping and morphological analyzing all the chromosomes of an organism according to the inherent morphological and structural characteristics. Karyotyping is an essential test to determine whether a cell line maintains a stable genotype.

Isoenzyme Analysis

Isoenzyme analysis is commonly used in conjunction with STP profiling or karyotyping. It is technically simple, rapid and robust. Electrophoretic banding patterns are used to examine the differences in the structure and mobility of various intracellular enzymes in different species.

Human Leukocyte Antigen (HLA) Typing

Human leukocyte antigens, a family of genes encoding the major histocompatibility complex (MHC) proteins, are one of the most polymorphic genes in human. HLP typing can be used to differentiate cell lines within a species.

Customized Cell Line Authentication Services

Our experienced scientists can customize the best solution according to our customers’ requirements. For particular cell types, such as tumor cells and stem cells, we have the ability to select more targeted approaches to achieve more comprehensive and accurate results.

Our Advantages

  • Extensive experience and state-of-the-art technique platforms.
  • Comprehensive services and customized solutions.
  • Rigorous cell line labeling and real-time monitoring.
  • Accurate results and reliable analysis.
  • Fast turnaround time and competitive price.

With years of experience in cell line authentication for human cell lines, mouse cell lines, monkey cell lines and some special cell lines, Creative Bioarray can be your reliable partner to help you ensure the reliability and reproducibility of your experimental results.

If you have any doubts about your cell line or experimental results, please feel free to contact us. We look forward to working with you.

References:

  1. Fusenig N E, et al. The need for a worldwide consensus for cell line authentication: experience implementing a mandatory requirement at the International Journal of Cancer. PLoS biology, 2017, 15(4): e2001438.
  2. Bian X, et al. A combination of species identification and STR profiling identifies cross-contaminated cells from 482 human tumor cell lines. Scientific reports, 2017, 7(1): 9774.
For research use only. Not for any other purpose.

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As a pioneer of biotechnology, Creative Bioarray has developed into one of the largest independent biotechnology companies in the world. We are committed to providing our customers with innovative high-quality products and services through discovering, developing, and manufacturing.

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