Human/Mouse Lefty Antibody

Product Code: CB0870

Catalog Number	Unit Size	Price
CB0870-25 ug	25 ug	Online Inquiry
CB0870-100 ug	100 ug	Online Inquiry

Description

Lefty was originally discovered while screening undifferentiated cell-specific cDNAs from P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned it the name "lefty". Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A (ebaf) and Lefty-B).
Lefty protein is novel TGF-beta ligand that functions as an antagonist of Nodal signaling. The Lefty contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers. In all species examined, lefty protein functions in patterning left-right asymmetry of the developing organ systems such as the heart and lung. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

Specification

Reactivity

Human, Mouse

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Source

Mouse myeloma cell line NS0-derived recombinant mouse Lefty-1, Leu22-Arg135, His6, Leu136-Pro368, Accession # Q64280

Specificity

Detects human and mouse Lefty in direct ELISAs and Western Blots.

Purification

Antigen Affinity-purified

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Small pack size is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Application

Western Blot

Alternative Names

AI450052;Ebaf;LEFTB;Lefty;LEFTY1;lefty-1;LEFTYB;RGD1561867;Stra3;Tgfb4;UNQ278/PRO317; Lefty

Entrez Gene IDs/Gene IDs

7044 (Human); 13590 (Mouse)

Endotoxin

<0.10 EU/μg by the LAL method.

Storage

At -20°C for one year. After reconstitution , at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

For research or further manufacturing use only. Not for use in humans.