Recombinant Human Thrombopoietin Protein, E. coli-derived, GMP grade

Product Code: G-GFC0615

Catalog Number	Unit Size	Price
G-GFC0615-50 ug	50 ug	Online Inquiry
G-GFC0615-1 mg	1 mg	Online Inquiry

Description

Thrombopoietin (TPO) is a 332 amino acid glycoprotein made primarily in the liver and act as the major physiological regulator of platelet production. TPO binds the TPO receptor, activates JAK and STAT pathways, thus stimulating megakaryocyte growth and platelet production. Additionally, further investigation uncovered that thrombopoietin is a critical cytokine promoting hematopoietic rebound after myeloablation and its transcripts are expressed by multiple cellular sources.

Specification

Source

E. coli-derived human Thrombopoietin/TPO protein, Ser22-Leu195
Produced in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Reactivity

Human

N-terminal Sequence

Ala-Ser22-Pro-Ala-Pro-Pro-Ala-(Cys)-Asp-Leu

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

18.7 kDa

SDS-PAGE

19 kDa, reducing conditions

Accession

NP_000451.1

Biological Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.05-0.5 ng/mL.The specific activity of Recombinant Human Thrombopoietin is >1 x 107 units/mg, which is calibrated against the human Thrombopoietin reference standard (NIBSC code: 03/124).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.