SuperEXP® Human CD4+CD25+CD127dim/- Regulatory T Cell Isolation Kit II

Product Code: RS1154

Catalog Number	Unit Size	Price
RS1154-for 1×10^9 total cells	for 1×10^9 total cells	Online Inquiry

Description

Creative Bioarray's CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit II is used to isolate CD4+CD25+CD127dim/– Treg cells from human PBMCs. The isolation is performed in a two-step procedure.

Specification

Species

Human

Application

Isolation of CD4+CD25+CD127dim/– regulatory T cells from human PBMCs for further phenotypical or functional characterization.

Detailed Separation Procedure

First, the isolation of CD4+CD25+CD127dim/– regulatory T cells was performed with a cocktail of biotinylated antibodies and Anti-Biotin Microbeads for the depletion of non-CD4+ and CD127high cells. In the second step, the flow-through fraction of pre-enriched CD4+CD127dim/– T cells is labeled with CD25 Microbeads for subsequent positive selection of CD4+CD25+CD127dim/– regulatory T cells.

For research or further manufacturing use only. Not for use in humans.