SuperEXP® Human CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit

Product Code: RS1153

Catalog Number	Unit Size	Price
RS1153-for 2×10^9 total cells	for 2×10^9 total cells	Online Inquiry

Description

Creative Bioarray's CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit is used to isolate CD45RA+ regulatory T cells (Treg) from human PBMCs in a two-step procedure.

Specification

Species

Human

Application

CD4+CD25+CD45RA+ regulatory T cells can be isolated from human PBMCs with this kit, for example, for further phenotypical or functional characterization and expansion.

Detailed Separation Procedure

The isolation is performed in a two-step procedure. First, non-CD4+ and memory T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin Microbeads. The labeled cells are subsequently depleted over a column. In the second step, the flow-through fraction of pre-enriched CD4+CD45RA+ T cells is labeled with CD25 Microbeads for subsequent positive selection of CD4+CD25+CD45RA+ T cells.

For research or further manufacturing use only. Not for use in humans.