SuperEXP® Human CD4+CD25+ Regulatory T Cell Isolation Kit

Human

The CD4+CD25+ Regulatory T Cell Isolation Kit has been used to investigate the role of CD4+CD25+ regulatory T cells in autoimmune diseases1,2, antitumor immunity3–6, allergy7, and in infectious diseases8. For further characterization, CD4+CD25+ regulatory T cells were isolated from patients suffering from rheumatoid arthritis1 or multiple sclerosis2, from acute T cell lymphoma patients4, from tumor biopsy specimens of non-Hodgkin lymphoma patients5, after dendritic cell vaccination9 as well as from patients with atopic dermatitis7.

The isolation is performed in a two-step procedure. First, non-CD4+ T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies against CD8, CD14, CD15, CD16, CD19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A) and Anti-Biotin Microbeads. The labeled cells are subsequently depleted over a Column. In the second step, the flow-through fraction of pre-enriched CD4+ T cells is labeled with CD25 Microbeads for subsequent positive selection of CD4+CD25+ regulatory T cells.