Recombinant Human IGF-I/IGF-1 Protein, E. coli-derived, GMP grade

Product Code: G-GFC0040

Catalog Number	Unit Size	Price
G-GFC0040-200 ug	200 ug	Online Inquiry
G-GFC0040-1 mg	1 mg	Online Inquiry

Description

Human insulin-like growth factor-I (IGF-I) is a single-chain 70-amino-acid polypeptide with structural homology to insulin and human IGF-II. IGF-I plays a key role in growth, differentiation, and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation.
IGF-I signals by binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signaling pathway. IGF-I also binds to each of the six IGF-binding proteins (IGFBPs). This binding also regulates the biological function of IGF-I. IGF-I is highly conserved across species, suggesting an important role for the peptide.

Specification

Source

E. coli-derived human IGF-I/IGF-1 protein, Gly49-Ala118
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Reactivity

Human

N-terminal Sequence

Gly-Pro-Glu-Thr-Leu-(Cys)-Gly-Ala-Glu-Leu

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions.

Molecular Mass (Predicted)

7.6 kDa

Accession

P05019.1

Biological Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-1.5 ng/mL.The specific activity of recombinant human IGF-I/IGF-1 is >1000 IU/mg, which is calibrated against the human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.