Recombinant Human BMP-2 Protein, CHO-derived, GMP grade

Product Code: G-GFC0003

Catalog Number	Unit Size	Price
G-GFC0003-10 ug	10 ug	Online Inquiry
G-GFC0003-50 ug	50 ug	Online Inquiry

Description

Bone morphogenetic protein 2 (BMP-2) is multi-functional growth factors that belong to the transforming growth factor beta (TGF beta) superfamily. BMP-2 is a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human BMP-2 protein, Gln283-Arg396
Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Reactivity

Human

N-terminal Sequence

Gln-Ala-Lys-His-Lys-Gln-Arg-Lys-Arg-Leu

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Molecular Mass (Predicted)

13 kDa (monomer)

SDS-PAGE

15-16, kDa, reducing conditions

Accession

P12643.1

Biological Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 40-200 ng/mL.The specific activity of recombinant human BMP-2 is >5.0 x 105 units/mg, which is calibrated against the human BMP-2 WHO Standard (NIBSC code: 93/574).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.