Insulin-Transferrin-Sodium Selenite-Ethanolamine-Sodium Pyruvate Media Supplement (100×) contains 0.2 mg/mL ethanolamine, 0.55 mg/mL human transferrin, 1.0 mg/mL recombinant human insulin, 12 mg/mL sodium pyruvate and 0.5 μg/mL sodium selenite. Insulin can promote the uptake of amino acids and glucose. Transferrin can bound to iron to nourish cultured cells. Sodium Selenite is an essential trace element. Ethanolamine is important for the proliferation of hybridoma cells. Sodium pyruvate is supplied as an additional energy source.
