Trypsin 0.25%-EDTA (1×)

Trypsin is mixture of proteases with digestive ability derived from porcine pancreas. It is widely used in cell culture research and cell biology studies for cell dissociation during routine cell culture passaging and tissue dissociation. The concentration of trypsin used for the successful isolation of cells mainly depends on the cell type and culture time.

Trypsin 0.25% is the most commonly used trypsin solution.

EDTA (Ethylenediaminetetraacetic acid) is an aminopolycarboxylic acid. It is a chelator of metal ions and can be used to neutralize calcium and magnesium ions. Since calcium and magnesium ions can enhance cell-to-cell adhesion, EDTA added to Trypsin can increase the enzymatic activity and facilitate release of individual cells.