Medium 199 was originally developed for the culture of primary explants. It needs to be supplemented with serum for the long term cultivation of cells. Medium 199 has broad applicability, especially in the cultivation of non-transformed cells. It has been widely used in virology and cultivation of primary cells
L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism.
Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.
Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.
