Ham's Nutrient Mixtures were originally designed as a serum-free formula for the clonal growth of CHO cells. Now Mam's F-12 can also be supplemented with serum to support the proliferation of various cells, such as rat prostate epithelial cells and chondrocytes.
L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism. Stable Glutamine with longer half-life in solution is an alternative to L-Glutamine.
Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.
Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.
