Ham's Nutrient Mixtures were originally designed as a serum-free formula for the clonal growth of CHO cells. Now Mam's F-10 can also be supplemented with serum to support the proliferation of various cells, such as COS-7, primary rat astrocytes, and rat prostate epithelial cells.
HEPES can help maintain pH level during cell culture.
L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism.
Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.
