DMEM/F-12, 15 mM HEPES, w/ L-Glutamine, w/o Sodium Bicarbonate, w/ Sodium Pyruvate, Powder

Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham

Catalog Number

M0059

Description

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 medium. This formulation combines DMEM's high concentrations of glucose, vitamins and amino acids with Ham's F-12's additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).

HEPES can help maintain pH level during cell culture.

L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism.

Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.

Specification

Product Size
1 L, 10 L, 10 × 1 L
Storage Conditions
2-8°C. Protected from light.
Shipping Conditions
Ambient
Sterility Testing
Non-Sterile
Quality and Performance Testing
Pass
Form
Powder
Phenol Red Indicator
Yes
Glucose
3.15 g/L
L-Glutamine
Yes
Sodium Bicarbonate
No
Sodium Pyruvate
0.055 g/L
HEPES
15 mM
For research or further manufacturing use only. Not for use in humans.

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