DMEM/F-12, 15 mM HEPES, w/o L-Glutamine, w/ Sodium Bicarbonate, w/ Sodium Pyruvate, w/o Phenol Red

Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham

Catalog Number

M0053

Description

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 medium. This formulation combines DMEM's high concentrations of glucose, vitamins and amino acids with Ham's F-12's additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).

HEPES can help maintain pH level during cell culture.

Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.

Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.

Specification

Product Size
500 mL, 1 L, 10 × 500 mL
Storage Conditions
2-8°C. Protected from light.
Shipping Conditions
Ambient
Sterility Testing
Pass
Quality and Performance Testing
Pass
Form
Liquid
Phenol Red Indicator
No
Glucose
3.15 g/L
L-Glutamine
No
Sodium Bicarbonate
Yes
Sodium Pyruvate
0.055 g/L
HEPES
15 mM
For research or further manufacturing use only. Not for use in humans.

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