DMEM/F-12, 15 mM HEPES, w/o L-Glutamine, w/ Sodium Bicarbonate, w/ Sodium Pyruvate, w/o Phenol Red
Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham
Description
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 medium. This formulation combines DMEM's high concentrations of glucose, vitamins and amino acids with Ham's F-12's additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).
HEPES can help maintain pH level during cell culture.
Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.
Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.
Specification
Product Size
500 mL, 1 L, 10 × 500 mL
Storage Conditions
2-8°C. Protected from light.
Shipping Conditions
Ambient
Quality and Performance Testing
Pass
Sodium Pyruvate
0.055 g/L
For research or further manufacturing use only. Not for use in humans.
