Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham
Catalog Number
M0047
Description
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 medium. This formulation combines DMEM's high concentrations of glucose, vitamins and amino acids with Ham's F-12's additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).
HEPES can help maintain pH level during cell culture.
L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism.
Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.
Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.
Specification
