DMEM/F-12, w/o HEPES, w/ L-Glutamine, w/ Sodium Bicarbonate, w/ Sodium Pyruvate

Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham

Catalog Number

M0039

Description

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 medium. This formulation combines DMEM's high concentrations of glucose, vitamins and amino acids with Ham's F-12's additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).

L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism.

Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.

Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.

Specification

Product Size
500 mL, 1 L, 10 × 500 mL
Storage Conditions
2-8°C. Protected from light.
Shipping Conditions
Ambient
Sterility Testing
Pass
Quality and Performance Testing
Pass
Form
Liquid
Phenol Red Indicator
Yes
Glucose
3.15 g/L
L-Glutamine
Yes
Sodium Bicarbonate
Yes
Sodium Pyruvate
0.055 g/L
HEPES
No
For research or further manufacturing use only. Not for use in humans.

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