DMEM/F-12, w/o HEPES, w/ L-Glutamine, w/ Sodium Bicarbonate, w/ Sodium Pyruvate
Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12 Ham
Description
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12 medium. This formulation combines DMEM's high concentrations of glucose, vitamins and amino acids with Ham's F-12's additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).
L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism.
Sodium pyruvate plays a role of carbon substitute in culture medium and participates in cell nutrition metabolism.
Sodium Bicarbonate is used to provide buffer system and adjust pH level during cell culture.
Specification
Product Size
500 mL, 1 L, 10 × 500 mL
Storage Conditions
2-8°C. Protected from light.
Shipping Conditions
Ambient
Quality and Performance Testing
Pass
Sodium Pyruvate
0.055 g/L
For research or further manufacturing use only. Not for use in humans.
