Synonym: DMEM/F-12, 1:1 Mixture, Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham
DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12 medium. This formulation combines DMEM’s high concentrations of glucose, vitamins and amino acids with Ham’s F-12’s additional components. Since this medium does not contain proteins, lipids, or growth factors, DMEM/F-12 usually requires supplementation with 10% Fetal Bovine Serum (FBS).
L-Glutamine is an essential amino acid for cell growth, which can be used as the energy source of cultured cells and participate in protein synthesis and nucleic acid metabolism. Stable Glutamine with longer half-life in solution is an alternative to L-Glutamine.