Human/Mouse Oct-3/4 Antibody, Unconjugated

Product Code: CB0890

Catalog Number	Unit Size	Price
CB0890-25 ug	25 ug	Online Inquiry
CB0890-100 ug	100 ug	Online Inquiry

Description

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif. It is expressed in totipotent embryonic stem and germ cells. When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program. The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.

Specification

Reactivity

Human, Mouse

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Label

Unconjugated

Source

E. coli-derived recombinant human Oct-3/4, Met1-Asn265 (Met262Leu), Accession # Q01860

Specificity

Detects human Oct-3/4 in direct ELISAs and Western Blots.

Purification

Antigen Affinity-purified

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Small pack size is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Application

Western Blot; Simple Western; Immunoprecipitation; Chromatin Immunoprecipitation (ChIP); Immunocytochemistry

Full Name

Octamer-binding Protein 3/4

Alternative Names

3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

Entrez Gene IDs/Gene IDs

5460 (Human); 18999 (Mouse)

Endotoxin

<0.10 EU/μg by the LAL method.

Storage

At -20°C for one year. After reconstitution , at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

For research or further manufacturing use only. Not for use in humans.