Human LIN-28B Antibody

Product Code: CB0876

Catalog Number	Unit Size	Price
CB0876-25 ug	25 ug	Online Inquiry
CB0876-100 ug	100 ug	Online Inquiry

Description

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming.、

Specification

Reactivity

Human

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Source

E. coli-derived recombinant human LIN-28B, Val83-Thr250, Accession # Q6ZN17

Specificity

Detects human LIN-28B in direct ELISAs and Western Blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human LIN-28A is observed.

Purification

Antigen Affinity-purified

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Small pack size is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Application

Western Blot; Immunohistochemistry

Full Name

LIN-28 Homolog B

Alternative Names

CSDD2; CSDD2protein lin-28 homolog B; FLJ16517; lin-28 homolog B (C. elegans); Lin-28.2; LIN28B; LIN-28B

Entrez Gene IDs/Gene IDs

389421 (Human); 380669 (Mouse); 689054 (Rat)

Endotoxin

<0.10 EU/μg by the LAL method.

Storage

At -20°C for one year. After reconstitution , at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

For research or further manufacturing use only. Not for use in humans.