Human Lefty-A Long and Short Isoforms Antibody

Product Code: CB0873

Catalog Number	Unit Size	Price
CB0873-25 ug	25 ug	Online Inquiry
CB0873-500 ug	500 ug	Online Inquiry

Description

Lefty was originally discovered while screening undifferentiated cell-specific cDNAs from P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned it the name "lefty". Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A (ebaf) and Lefty-B).
Lefty protein is novel TGF-beta ligand that functions as an antagonist of Nodal signaling. The Lefty contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers. In all species examined, lefty protein functions in patterning left-right asymmetry of the developing organ systems such as the heart and lung. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

Specification

Reactivity

Human

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Clone Number

182525

Source

Mouse myeloma cell line NS0-derived recombinant human Lefty-A, Phe78-Pro366, Accession # O00292.2

Specificity

Detects both long and short forms of recombinant human (rh) Lefty-A in direct ELISAs and Western Blots. No cross-reactivity with rhArtemin, recombinant mouse (rm) Artemin, recombinant drosophila DPP, rhGDNF, rhLAP, recombinant rat MIS, rhNeurturin, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, or rmLefty-1. The cross-reactivity of this antibody with rhLefty-B (96% amino acid sequence homology) was not determined.

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Small pack size is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Application

Western Blot

Alternative Names

EBAFTGF-beta-4; endometrial bleeding associated factor; Endometrial bleeding-associated factor; Lefta; LEFTAProtein lefty-A; left-right determination factor 2; Left-right determination factor A; LEFTY2; LeftyA; Lefty-A; LEFTYATransforming growth factor beta-4; MGC46222; Protein lefty-2; TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily)

Entrez Gene IDs/Gene IDs

7044 (Human)

Endotoxin

<0.10 EU/μg by the LAL method.

Storage

At -20°C for one year. After reconstitution , at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

For research or further manufacturing use only. Not for use in humans.