Human CHD1 Antibody, Unconjugated

Product Code: CB0832

Catalog Number	Unit Size	Price
CB0832-25 ug	25 ug	Online Inquiry
CB0832-100 ug	100 ug	Online Inquiry

Description

CHD1 belongs to the SNF2/RAD54 helicase family. CHD1 is an ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. CHD1 regulates polymerase II transcription. It is required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step.
CHD1 regulates negatively DNA replication. It is required to maintain a specific chromatin configuration across the genome. It is associated with histone deacetylase (HDAC) activity. CHD1 is required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. It functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. It is required for maintaining open chromatin and pluripotency in embryonic stem cells.

Specification

Reactivity

Human

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Clone Number

677616

Label

Unconjugated

Source

E. coli-derived recombinant human CHD1, Lys1531-Thr1710, Accession # O14646

Specificity

Detects human CHD1 in direct ELISAs and Western Blots. In direct ELISAs, no cross-reactivity with recombinant human CHD1L, 5, or 7 is observed.

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Small pack size is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Application

Western Blot; Immunocytochemistry; Knockout Validated

Full Name

Chromodomain Helicase DNA Binding Protein 1

Alternative Names

ATP-dependent helicase CHD1; CHD1; CHD-1; chromodomain helicase DNA binding protein 1; chromodomain-helicase-DNA-binding protein 1; DKFZp686E2337; EC 3.6.1; EC 3.6.4.12

Entrez Gene IDs/Gene IDs

1105 (Human); 12648 (Mouse)

Endotoxin

<0.10 EU/μg by the LAL method.

Storage

At -20°C for one year. After reconstitution , at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

For research or further manufacturing use only. Not for use in humans.