Fixable Viability Dye UV 450

Fixable Viability Dye UV 450 is a fluorescent cell viability dye used to discriminate viable from non-viable mammalian cells in flow cytometry applications. The dye binds intracellular and cell surface amine groups. Non-viable cells without of membrane integrity will be labeled with significantly more dye than healthy cells in the same sample. The degree of labeling will be preserved through fixation, permeabilization, and antibody incubation. The dye can be excited by the UV laser at 355 nm and it has a fluorescence emission maximum of 450 nm.