Recombinant Human Thrombopoietin Protein, E. coli-derived

Product Code: GFC0615

Catalog Number	Unit Size	Price
GFC0615-10 ug	10 ug	Online Inquiry
GFC0615-50 ug	50 ug	Online Inquiry
GFC0615-100 ug	100 ug	Online Inquiry
GFC0615-500 ug	500 ug	Online Inquiry
GFC0615-1 mg	1 mg	Online Inquiry

Description

Thrombopoietin (TPO) is a 332 amino acid glycoprotein made primarily in the liver and act as the major physiological regulator of platelet production. TPO binds the TPO receptor, activates JAK and STAT pathways, thus stimulating megakaryocyte growth and platelet production. Additionally, further investigation uncovered that thrombopoietin is a critical cytokine promoting hematopoietic rebound after myeloablation and its transcripts are expressed by multiple cellular sources.

Specification

Source

E. coli-derived human Thrombopoietin/TPO protein, Ser22-Leu195

Reactivity

Human

N-terminal Sequence

Ala-Ser22

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

18.7 kDa

SDS-PAGE

19 kDa, reducing conditions

Accession

NP_000451

Biological Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.05-0.5 ng/mL.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.