Recombinant Human Thrombopoietin Protein, NS0-derived

Product Code: GFC0614

Catalog Number	Unit Size	Price
GFC0614-10 ug	10 ug	Online Inquiry
GFC0614-25 ug	25 ug	Online Inquiry
GFC0614-100 ug	100 ug	Online Inquiry

Description

Thrombopoietin (TPO) is a 332 amino acid glycoprotein made primarily in the liver and act as the major physiological regulator of platelet production. TPO binds the TPO receptor, activates JAK and STAT pathways, thus stimulating megakaryocyte growth and platelet production. Additionally, further investigation uncovered that thrombopoietin is a critical cytokine promoting hematopoietic rebound after myeloablation and its transcripts are expressed by multiple cellular sources.

Specification

Source

Mouse myeloma cell line, NS0-derived human Thrombopoietin/TPO protein, Ser22-Gly353

Reactivity

Human

N-terminal Sequence

Ser22

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

35 kDa

SDS-PAGE

80-90 kDa, reducing conditions

Accession

P40225

Biological Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 5-15 ng/mL.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.