Recombinant Human TGF-beta 1 Protein, Avi-tag

Product Code: GFC0613

Catalog Number	Unit Size	Price
GFC0613-25 ug	25 ug	Online Inquiry

Description

Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. Recombinant human TGF-β1 is used for the cultivation of embryonic stem cells and induced pluripotent stem cells, but can also promote Th17 and Treg differentiation of T cells.

Specification

Source

Human embryonic kidney cell, HEK293-derived human TGF-beta 1 protein, Avi-tag, Ala279-Ser390

Reactivity

Human

N-terminal Sequence

Gly of Avi-tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

15 kDa

SDS-PAGE

12-16 kDa, under reducing conditions

Accession

NP_000651.3

Biological Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human TGF-beta RII Fc Chimera is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human TGF-beta 1 Avi-tag binds with an ED50 of 0.80-6.40 ng/mL.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.