Recombinant Human PDGF-BB Protein, Biotinylated

Product Code: GFC0605

Catalog Number	Unit Size	Price
GFC0605-10 ug	10 ug	Online Inquiry

Description

PDGF (Platelet-derived growth factor) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues.
PDGF-BB is a dimeric protein composed by two identical B subunits, it belongs to the PDGF family together with the two receptors PDGFRα and PDGFRβ. PDGF-BB binds PDGFRβ with the highest affinity. It regulates mesenchymal cell growth, morphogenesis, and migration, especially during angiogenesis and glial cell proliferation.

Specification

Source

E. coli-derived human PDGF-BB protein, Ser82-Thr190

Reactivity

Human

Purity

>95%, by SDS-PAGE with silver staining.

Molecular Mass (Predicted)

12 kDa (unlabeled)

SDS-PAGE

12 kDa, reducing conditions

Accession

P01127.1

Biological Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.

Endotoxin

<1.0 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.