Recombinant Human PDGF-BB Protein, GMP grade

Product Code: G-GFC0602

Catalog Number	Unit Size	Price
G-GFC0602-50 ug	50 ug	Online Inquiry
G-GFC0602-1 mg	1 mg	Online Inquiry

Description

PDGF (Platelet-derived growth factor) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues.
PDGF-BB is a dimeric protein composed by two identical B subunits, it belongs to the PDGF family together with the two receptors PDGFRα and PDGFRβ. PDGF-BB binds PDGFRβ with the highest affinity. It regulates mesenchymal cell growth, morphogenesis, and migration, especially during angiogenesis and glial cell proliferation.

Specification

Source

E. coli-derived human PDGF-BB protein, Ser82-Thr190
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Reactivity

Human

N-terminal Sequence

Ser-Leu-Gly-Ser-Leu-Thr-Ile-Ala-Glu-Pro

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions.

Molecular Mass (Predicted)

12.3 kDa (monomer)

Accession

P01127

Biological Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.The specific activity of recombinant human PDGF-BB is >7.5 x 105 IU/mg, which is calibrated against the human PDGF-BB WHO International Standard (NIBSC code: 94/728).

Endotoxin

<0.01 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.