Recombinant Human FGF-17 Protein

Product Code: GFC0590

Catalog Number	Unit Size	Price
GFC0590-25 ug	25 ug	Online Inquiry

Description

Fibroblast Growth Factor 17 (FGF-17) is a heparin-binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.

Specification

Source

E. coli-derived human FGF-17 protein, Thr23-Thr216, with and without an N-terminal Met

Reactivity

Human

N-terminal Sequence

Met & Thr23

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

22.6 kDa

SDS-PAGE

21.5 & 22.6 kDa, reducing conditions

Accession

O60258

Biological Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 100‑500 ng/mL, in the presence of 1 µg/mL heparin, in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002) and 15‑60 ng/mL, in the presence of 1 µg/mL heparin, when measured by 3H-thymidine incorporation.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.