Recombinant Human Dkk-1 Protein, N-Terminal Fragment

Product Code: GFC0587

Catalog Number	Unit Size	Price
GFC0587-50 ug	50 ug	Online Inquiry

Description

Dickkopf related protein 1 (DKK1) is a chemokine belonging to the DKK protein family, which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally thought to be a Xenopus head-forming molecule that acts as an antagonist of Wnt signaling.
DKK1 not only functions as a head inducer during development, but also regulates joint remodeling and bone formation, which indicate sits role in the pathogenesis of rheumatoid arthritis and multiple myeloma.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human Dkk-1 protein, Thr32-Ser141, with a C-terminal 6-His tag

Reactivity

Human

N-terminal Sequence

Thr32, Ser35 & Ser39

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

12 kDa

SDS-PAGE

13 - 21 kDa, reducing conditions

Accession

O94907

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Dkk‑1 N-Terminal Fragment is coated at 1 μg/mL, Mouse CKAP4/p63 (Catalog # 9734-CK) binds with an ED50 of 9-54 ng/mL.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.