Recombinant Human Dkk-1 Protein, Biotinylated

Product Code: GFC0586

Catalog Number	Unit Size	Price
GFC0586-10 ug	10 ug	Online Inquiry

Description

Dickkopf related protein 1 (DKK1) is a chemokine belonging to the DKK protein family, which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally thought to be a Xenopus head-forming molecule that acts as an antagonist of Wnt signaling.
DKK1 not only functions as a head inducer during development, but also regulates joint remodeling and bone formation, which indicate sits role in the pathogenesis of rheumatoid arthritis and multiple myeloma.

Specification

Source

Spodoptera frugiperda, Sf21 (baculovirus)-derived human Dkk-1 protein, Thr32-His266

Reactivity

Human

N-terminal Sequence

Thr32

Purity

>95%, by SDS-PAGE with silver staining.

Molecular Mass (Predicted)

26 kDa (unlabeled)

SDS-PAGE

33-38 kDa, reducing conditions

Accession

O94907

Biological Activity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Biotinylated Recombinant Human Dkk-1 inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt‑3a . The ED50 for this effect is 10-60 ng/mL.

Endotoxin

<1.0 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.