Recombinant Human BMP-2/BMP-6 Heterodimer Protein

Product Code: GFC0584

Catalog Number	Unit Size	Price
GFC0584-10 ug	10 ug	Online Inquiry

Description

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, and BMP-2 are members of the BMP family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta) superfamily.
Both BMP-2 and BMP-6 induce osteogenic and chondrogenic differentiation in mesenchymal stem cells. Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers. BMP-2/6 heterodimers also show increased activity at inducing trophectodermal and endodermal differentiation of embryonic stem cells compared to either homodimer.

Specification

Source

E. coli-derived human BMP-2/BMP-6 Heterodimer protein
Human BMP-2 (Ala284 - Arg396), with an N-terminal Met
Human BMP-6 (Gln382 - His513), with an N-terminal Met

Reactivity

Human

N-terminal Sequence

Ala284 (BMP-2) & Met (BMP-6)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

12.8 kDa (BMP-2) & 15 kDa (BMP-6)

SDS-PAGE

11 kDa & 14 kDa, reducing conditions

Accession

P12643 (BMP-2) & P22004 (BMP-6)

Biological Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 4-20 ng/mL.

Endotoxin

<0.01 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.