Recombinant Human Pro-EGF Protein, aa 21-1023

Product Code: GFC0577

Catalog Number	Unit Size	Price
GFC0577-25 ug	25 ug	Online Inquiry

Description

Epidermal growth factor (EGF) is a member of the EGF-family and plays an important role in the regulation of cell growth, proliferation, and differentiation through binding to its receptor EGFR. Recombinant human EGF is optimized for use in cell culture, differentiation studies, and functional assays.

Specification

Source

Mouse myeloma cell line, NS0-derived human EGF protein, Met1-Arg1023, with a C-terminal 6-His tag

Reactivity

Human

N-terminal Sequence

Ser21

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

112 kDa

SDS-PAGE

138-145 kDa, reducing conditions

Accession

CAA28240

Biological Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1-5 ng/mL.

Endotoxin

<0.01 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.