Recombinant Human EGF Protein, Animal Component-Free

Product Code: GFC0576

Catalog Number	Unit Size	Price
GFC0576-200 ug	200 ug	Online Inquiry
GFC0576-1 mg	1 mg	Online Inquiry

Description

Epidermal growth factor (EGF) is a member of the EGF-family and plays an important role in the regulation of cell growth, proliferation, and differentiation through binding to its receptor EGFR. Recombinant human EGF is optimized for use in cell culture, differentiation studies, and functional assays.

Specification

Source

E. coli-derived human EGF protein, Asn971-Arg1023, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

Reactivity

Human

N-terminal Sequence

Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

6 kDa

Accession

P01133

Biological Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20‑100 pg/mL.The specific activity of Recombinant Human EGF is >8.0 x 105 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.