Recombinant Human EGF Protein

Product Code: GFC0575

Catalog Number	Unit Size	Price
GFC0575-200 ug	200 ug	Online Inquiry
GFC0575-1 mg	1 mg	Online Inquiry

Description

Epidermal growth factor (EGF) is a member of the EGF-family and plays an important role in the regulation of cell growth, proliferation, and differentiation through binding to its receptor EGFR. Recombinant human EGF is optimized for use in cell culture, differentiation studies, and functional assays.

Specification

Source

E. coli-derived human EGF protein, Asn971-Arg1023, with an N-terminal Met

Reactivity

Human

N-terminal Sequence

Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

6 kDa

SDS-PAGE

6 kDa, reducing conditions

Accession

P01133

Biological Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.