Recombinant Human Wnt-3a Protein, High Purity

Product Code: GFC0574

Catalog Number	Unit Size	Price
GFC0574-10 ug	10 ug	Online Inquiry

Description

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctional factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein, Ser19-Lys352

Reactivity

Human

N-terminal Sequence

Ser19

Purity

>90%, by SDS-PAGE with silver staining.

Molecular Mass (Predicted)

37.4 kDa

SDS-PAGE

40 kDa, reducing conditions

Accession

NP_149122

Biological Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1.5-7.5 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.Optimal concentrations should be determined by each laboratory for each application.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.