Recombinant Human Wnt-3a Protein

Product Code: GFC0573

Catalog Number	Unit Size	Price
GFC0573-10 ug	10 ug	Online Inquiry
GFC0573-500 ug	500 ug	Online Inquiry

Description

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctional factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein, Ser19-Lys352

Reactivity

Human

N-terminal Sequence

Ser19

Purity

>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

37.4 kDa

SDS-PAGE

40 kDa, reducing conditions

Accession

NP_149122

Biological Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.Optimal concentrations should be determined by each laboratory for each application.

Endotoxin

<1.0 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.