Recombinant Human Latent TGF-beta 1 Protein, CHO-derived

Product Code: GFC0570

Catalog Number	Unit Size	Price
GFC0570-5 ug	5 ug	Online Inquiry
GFC0570-25 ug	25 ug	Online Inquiry

Description

Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. Recombinant human TGF-β1 is used for the cultivation of embryonic stem cells and induced pluripotent stem cells, but can also promote Th17 and Treg differentiation of T cells.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein, Leu30-Ser390

Reactivity

Human

N-terminal Sequence

Leu30 (LAP) & Ala279 (Mature)

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

28.5 kDa (LAP) & 12.8 kDa (Mature), Monomer

SDS-PAGE

36-42 kDa & 11 kDa, reducing conditions80-95 kDa & 24 kDa, non-reducing conditions

Accession

P01137

Biological Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 10-70 ng/mL before acid activation and 0.15‑0.9 ng/mL after acid activation.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.