Recombinant Human Nodal Protein

Product Code: GFC0569

Catalog Number	Unit Size	Price
GFC0569-25 ug	25 ug	Online Inquiry

Description

Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.

Specification

Source

E. coli-derived human Nodal protein, His238-Leu347, with an N-terminal Met

Reactivity

Human

N-terminal Sequence

Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Molecular Mass (Predicted)

12.9 kDa (monomer)

Accession

Q96S42

Biological Activity

Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al. (2001) Mol. Cell. 7:949. Approximately 100 ng/mL of Recombinant Human Nodal can effectively induce Smad2 phosphorylation.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.