Recombinant Human FGF basic/FGF2/bFGF, 145 aa, TC Grade

Product Code: GFC0566

Catalog Number	Unit Size	Price
GFC0566-1 mg	1 mg	Online Inquiry

Description

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.

Specification

Source

E. coli-derived human FGF basic/FGF2/bFGF protein, Ala144-Ser288

Reactivity

Human

N-terminal Sequence

Ala144

Molecular Mass (Predicted)

16 kDa

Accession

NP_001997

Biological Activity

The protein is used at 20 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells over 3 passages. Cells were cultured in N2 plus medium and supplemented with FGF basic every day. Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.3-3 ng/mL.

Endotoxin

<0.01 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.