Recombinant Human Latent Activin A Protein, CHO-derived

Product Code: GFC0558

Catalog Number	Unit Size	Price
GFC0558-25 ug	25 ug	Online Inquiry

Description

Activin A is a TGFβ family growth factor used frequently for the maintenance of pluripotency in induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture. Activin A is a homodimer of two beta A chains and is not biologically active until the N terminal propeptide is cleaved from each. Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human Activin A protein, Ser21-Ser426

Reactivity

Human

N-terminal Sequence

Ser21 (pro) & Gly311 (mature)

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

32 kDa (pro) & 13 kDa (mature)

SDS-PAGE

13-16 kDa (mature) and 39 - 46 kDa (pro), reducing conditions

Accession

P08476

Biological Activity

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.600‑3.60 ng/mL.

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.