Recombinant Human/Mouse/Rat Activin A Protein, GMP grade

Product Code: G-GFC0553

Catalog Number	Unit Size	Price
G-GFC0553-10 ug	10 ug	Online Inquiry
G-GFC0553-50 ug	50 ug	Online Inquiry
G-GFC0553-1 mg	1 mg	Online Inquiry

Description

Activin A is a TGFβ family growth factor used frequently for the maintenance of pluripotency in induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) culture. Activin A is a homodimer of two beta A chains and is not biologically active until the N terminal propeptide is cleaved from each. Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived Activin A protein, Gly311-Ser426
Manufactured and tested under cGMP guidelines.

Reactivity

Human/Mouse/Rat

N-terminal Sequence

Gly311-Leu-Glu-(Cys)-Asp-Gly-Lys-Val-Asn-Ile

Purity

>97%, by SDS-PAGE with silver staining.

Molecular Mass (Predicted)

13 kDa (monomer)

SDS-PAGE

14 kDa, reducing conditions24 kDa, non-reducing conditions

Accession

P08476.2

Biological Activity

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2-1.2 ng/mL.The specific activity of Recombinant Human/Mouse/Rat Activin A is >1000 units/mg, which is calibrated against the human Activin A WHO reference standard (NIBSC code: 91/626).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.